Isolation Of Canine Adipose Derived Mesenchymal Stem Cells From Falciform Tissue Obtained Via Laparoscopic Morcellation

Download or read book Isolation of Canine Adipose-derived Mesenchymal Stem Cells from Falciform Tissue Obtained Via Laparoscopic Morcellation written by Christine DePompeo and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Objective: To evaluate feasibility of stem cell isolation from falciform fat harvested via laparoscopic morcellation. Study Design: Pilot study. Animals: 11 client-owned dogs. Methods: Falciform was harvested traditionally via laparotomy, and laparoscopically via tissue morcellation. Harvested tissue was processed to obtain a stromal vascular fraction (SVF) using a commercially available adipose tissue dissociation kit. Cells were subsequently labelled for CD90, CD45, and CD44 cell surface antigens using magnetic-activated cell sorting (MACS) and fluorescence-activate cell sorting (FACS) flow cytometry. CD90 positive cells were quantified and their viability assessed using a hemocytometer and trypan blue exclusion test of cell viability. Results: No peri-operative complications occurred in patients undergoing laparoscopic morcellation. Laparoscopically and traditionally harvested samples yielded an average of 0.39 (+/- 0.1) x 106 and 0.33 (+/- 0.1) x 106 CD90+ cells per 10 million SVF cells, respectively. CD90+ cell viability following MACS was 89% (+/- 11%) for morcellated and 86% (+/- 7%) for traditionally harvested samples. Neither CD90+ cell quantity nor viability was significantly different between samples obtained via traditional laparotomy versus laparoscopic morcellation (p = 0.38 and p = 0.63, respectively). Populations of CD90+ cells isolated using each harvest technique had similar CD44 and CD45 expression profiles. Conclusion: Viable populations of CD90+ cells with similar CD44/CD45 expression profiles could be isolated from laparoscopically morcellated and traditionally harvested falciform tissue. No appreciable morbidity was associated with laparoscopic falciform morcellation. Clinical Significance: Laparoscopic morcellation is a safe and effective minimally invasive means of falciform tissue harvest for AD-MSC isolation.