Microorganisms In Sea Ice Melt Pools As A Source Of Ultra Violet Radiation Absorbing Metabolites

Download or read book Microorganisms in Sea Ice Melt Pools as a Source of Ultra-violet Radiation Absorbing Metabolites written by Amanda Thomas and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Natural products have many uses in today's society, from disease therapeutics to cosmetic applications. One such application of natural products is use as an active ingredient in commercial sunscreens. Ultra-violet (UV) radiation exposure can result in a range of harmful side effects from a minor burn to the induction of melanoma. Due to the hazards associated with UV exposure, there is a need for safe and effective natural sunscreens. Microbes are known to produce structurally diverse natural products with greatly varied functions. One potential role of microbial natural products is to act as UV protectants for the producing organism. For this thesis we wanted to describe the cultivable microbial community of sea ice melt pools to determine if microbes in this habitat are resistant to UVB radiation, and if their mechanism of resistance was via the production of UV protectants. Thus, microbes living in high UV intense habitats are of interest for this thesis. One such habitat is sea ice melt pools in Canada's Arctic. During the early summer months the sea ice begins to melt forming melt pools. Due to the constant sun exposure, coupled with the reflective property of the ice, microbes present in these pools endure extreme levels of UV radiation. Microorganisms have three mechanisms in which they can survive exposure to UV radiation. They can produce spores, have DNA repair mechanisms, or they can produce UV-absorbing metabolites. With this knowledge it was hypothesized that microbes living in these melt pools would be resistant to UV radiation via the production of UV-absorbing metabolites. Water samples were collected from sea ice melt pools in Nunavut and the cultivable microbial community was identified via sequencing of the 16S rRNA gene (bacteria) and the ITS/28S rRNA genes (fungi). Phylogenetic analysis revealed that the microbes belonged to 26 different species. Of these 26 species two of the organisms, Frigidiomyces aurantiacum and Polaromyces triangulaformis, were discovered in this study and were described during the course of the research. Each of the 26 organism's temperature growth range, nutrient requirements, ability to survive a freeze thaw cycle, and resistance to UVB radiation were determined. Post exposure to UVB radiation, compounds produced by each organism was extracted to determine if UV-absorbing metabolites were being produced. The crude extracts were then analyzed using HPLCHRMS. Of the 26 organisms, seven were true psychrophiles, 17 could survive with minimal nutrients, all of the organisms tested remained viable after a single freeze-thaw cycle, and 20 were resistant to exposure to UVB radiation. HPLC-HRMS analysis of the crude extracts revealed that four strains produced mycosporines or mycosporine-like amino acids. One bacterium, Rhodococcus sp. RKAT245, produced a single mycosporine-like amino acid, shinorine. From the fungal library, Bulleromyces albus produced mycosporine-glutaminol, Dioszegia sp. RKAT 238 produced mycosporineglutaminol, mycosporine-glutaminol-glucoside, and mycosporine-glutamicol-glucoside, while Frigidiomyces aurantiacum produced mycosporine-glutaminol-glucoside.