Microrna Regulation Of Tumorigenic Pathways In Inflammatory Bowel Disease Associated Cancer

Download or read book MicroRNA Regulation of Tumorigenic Pathways in Inflammatory Bowel Disease-associated Cancer written by Ziad M. Kanaan and published by . This book was released on 2010 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Inflammatory bowel diseases (IBD) consist of Crohn's disease (CD) and ulcerative colitis (UC). IBD patients have an increased risk of colorectal cancer (CRC) development. MicroRNAs (miRNAs) are small non-protein-coding RNAs that play important roles in CRC oncogenesis. We aim to identify differentially expressed miRNAs in IBD-associated CRC progression with study of their possible effect on tumorigenic pathways such as the p53 pathway. Eighteen formalin-fixed paraffin-embedded (FFPE) colonic tissue specimens representing IBD-CRC progression from six IBD (4 CD and 2 UC) patients were utilized in this study. Pathologist-guided laser capture micro-dissection was performed to isolate cell populations of normal, dysplastic and cancerous epithelial cells from each patient. miRNA detection was performed using reverse transcription polymerase chain reaction (RT-PCR) arrays in triplicate and data was subjected to "bioinformatics pathway analysis" to understand the effect of differentially expressed miRNAs upon the p53 pathway. We were able to track CRC progression in all IBD patients, from normal tissue to dysplasia and eventually cancer. Five miRNAs were commonly down-regulated (miR-193b, miR-373, let-7e, miR-15b, and miR-372) (p