Isolation Characterization And In Vitro Proliferation Of Canine Bone Marrow Adipose Tissue Muscle And Periosteum Derived Mesenchymal Stem Cells

Download or read book Isolation, Characterization, and in Vitro Proliferation of Canine Bone Marrow, Adipose Tissue, Muscle, and Periosteum-derived Mesenchymal Stem Cells written by Agatha H. Kisiel and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Procedures: Characterization of MSCs was based on their plastic adherence and morphology, immunofluorescence of MSC-associated cell surface markers, and expression of pluripotency-associated transcription factors. Morphological and histochemical methods were used to evaluate differentiation of MSCs cultured in adipogenic, osteogenic, and chondrogenic media. Passage one MSCs, cultured in triplicate, were counted at 24, 48, 72, and 96 hours to determine tissue specific-MSC proliferative capacity. Mesenchymal stem cell yield/gram of tissue was calculated for confluent passage one MSCs.