Low Cost Methods For Molecular Characterization Of Mutant Plants

Download or read book Low-Cost Methods for Molecular Characterization of Mutant Plants written by Bradley J. Till and published by Springer. This book was released on 2015-05-08 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.