Response And Physiology Of Grafted Glyphosate Resistant And Conventional Soybean Glycine Max To Glyphosate And Efficacy Of Bicyclopyrone In Vegetables

Download or read book Response and Physiology of Grafted Glyphosate-resistant and Conventional Soybean (glycine Max) to Glyphosate, and Efficacy of Bicyclopyrone in Vegetables written by Yin Chen and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques to improve specialty crop herbicide tolerance, and identifying new methods of weed control are needed to help secure the future of vegetable production. My dissertation research addressed both concerns. First, I used soybean as model plant to investigate response and physiology of grafted glyphosate-resistant (RR) and conventional (CN) soybean plants to glyphosate. Glyphosate (0.28, 0.84 and 1.68 kg ae ha-1) was applied to CN/CN (scion/rootstock), CN/RR, RR/CN (only in growth stage experiment) and RR/RR grafted plants that had been generated using CN and RR genotypes. Variables tested included three growth stages (3, 6 and 10-leaf stages), nine CN/RR genotype combinations, and two growing environments (day/night thermoperiods of 28/22 C and 24/18 C). Small CN/RR plants (65%) and medium-sized plants (50%) were injured more than large plants (40%) 34 days with 0.84 kg ae ha-1 of glyphosate. Genotype of the scion had a greater effect on glyphosate tolerance compared to rootstock. Fewer leaves produced on CN/RR construct 5388/9392 under the warmer day/night temperatures following treatment with glyphosate. Seventy-two hours after treatment (HAT), 35% less shikimate was found in CN/RR compared to CN/CN plants. No cp4-epsps mRNA was detected in leaves of CN/RR plants, but a very small amount of CP4-EPSPS protein (=0.004% of that in RR leaf) was detected in the CN/RR leaves. More foliar-applied 14C-glyphosate and derivative was translocated to the root system of CN/RR compared to CN/CN. These data indicate that translocation of glyphosate from the CN scion to the RR rootstock is a major contributor to the partial tolerance to glyphosate observed in CN/RR transgrafted soybeans. Second, to aid in development of new weed control methods for vegetables, I assessed the response of several vegetable crops as well as three important weed species to the new herbicide bicyclopyrone. Onion, carrot, radish and dill were relatively tolerant of pre-emergence (PRE) and post-directed (POSTDIR) bicyclopyrone at 37.5 and 50 g ai ha-1. In contrast, bicyclopyrone post-emergence (POST) induced severe injury. Greenhouse experiments further examined the effect of soil type and variety on the response of onion, carrot and leek to 0, 12.5, 25, 50 and 100 g ha-1 of bicyclopyrone applied PRE. All test crops were uninjured by bicyclopyrone PRE when grown in muck soil, whereas all crops grown in sand + Pro-Mix BXTM blend were injured. Control of hairy galinsoga (Galinsoga quadriradiata Cav.), common purslane (Portulaca oleracea L.) and prostrate pigweed (Amaranthus blitoides S. Wats.) treated with bicyclopyrone POST or PRE was assessed in different soils and with each weed at three stages of growth. POST bicyclopyrone at 37.5 and 50 g ha-1 controlled hairy galinsoga and small common purslane plants (¿ 80% injury).. Hairy galinsoga was well controlled (80% injury) when growing in a Wooster silt loam and in a Pro-Mix BXTM 2:3 v/v blend. Common purslane was injured by PRE application to the muck soil and was well controlled in the Wooster silt loam and Pro-Mix BXTM blend. Bicyclopyrone POST and PRE did not control prostrate pigweed in either soil type or at any growth stage.